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A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
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JoVE Journal Neuroscience
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

DOI:
10.3791/2807-v

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04:35 min

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July 26, 2011

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, ,
1Department of Molecular & Human Genetics,Baylor College of Medicine (BCM), 2Precisionary Instruments Inc., 3Departments of Molecular & Human Genetics and Neuroscience,Baylor College of Medicine (BCM), 4Jan and Dan Duncan Neurological Research Institute,Texas Children's Hospital

Chapters

  • 00:05Title
  • 01:01Agarose Embedding of Fixed Mouse Brain Tissue
  • 01:55Sectioning the Embedded Brain Tissue Using the Compresstome
  • 02:33Optical Clearing of the Brain Tissue Sections
  • 03:14Mounting the Brain Tissue Sections for Imaging
  • 03:53Epifluorescent and Confocal Microscopy of Intact Thick Brain Slices
  • 04:16Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a rapid and simple method to image fluorescently labeled cells in semi-thick brain slices. By fixing, slicing, and optically clearing brain tissue we describe how standard epifluorescent or confocal imaging can be used to visualize individual cells and neuronal networks within intact nervous tissue.

Tags

High-resolution Fluorescence ImagingSemi-thick Brain SlicesNeuroscienceNeuroanatomical MapsMolecular GeneticsLight MicroscopyNeuronal MorphologiesMolecular And Cellular MakeupTransgenic And Gene Targeting TechnologiesFluorescent ProteinsMarine InvertebratesCell Type-specific Promoter ActivityBrain Structure And Function

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