Journal
/
Immunology and Infection
/
Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

DOI:
10.3791/2820-v

•

11:29 min

•

December 03, 2011

•

, , , , ,
1Laboratory of Water and Food Viral Pollution, Department of Microbiology, Faculty of Biology,University of Barcelona

Chapters

  • 00:05Title
  • 00:11Introduction
  • 01:56Collection of Water Samples
  • 02:45Viral Concentration by Organic Flocculation
  • 06:10Nucleic Acid Extraction and Quantification of Viruses
  • 10:46Conclusion

Summary

Automatic Translation

Automatic Translation

The study describes a cost-effective method for the identification of the source of fecal/urine contamination or contamination by nitrates in water using qPCR for the specific quantification of human/porcine/bovine DNA viruses, adenoviruses and polyomaviruses, proposed as MST tools.

Tags

Microbial Source TrackingCost-effective MethodSpecific Human VirusesSpecific Animal VirusesViral IndicatorsFecal ContaminationWater QualityMolecular AssaysAdenovirusesPolyomavirusesQuantitative PCR (qPCR)Porcine AdenovirusesBovine PolyomavirusesWater Sample AnalysisNitrates

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2820?article_type=v

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
check_url/2820?article_type=v

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model
check_url/2820?article_type=v

Using Luciferase to Image Bacterial Infections in Mice
check_url/2820?article_type=v

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
check_url/2820?article_type=v

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
check_url/2820?article_type=v

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
check_url/2820?article_type=v

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity
check_url/2820?article_type=v

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
check_url/2820?article_type=v

Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
check_url/2820?article_type=v

VirWaTest, A Point-of-Use Method for the Detection of Viruses in Water Samples

Read Article