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Detección del virus infeccioso de campo recogidos por los mosquitos del ensayo cultivo celular Vero
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Chapters
- 00:05Title
- 01:13Mosquito Sorting and Collection
- 02:39Homogenization of Mosquitoes
- 03:25Preparation of Vero Cells
- 04:49Inoculation of Vero cells with Mosquito Pool Homogenates
- 05:38Screening Vero Cells for Virus Growth
- 06:40Viruses Detected in Field-collected Mosquitoes by Vero Cell Culture Assay
- 07:05Conclusion
Se describe un método para procesar y la pantalla de campo recogidos mosquitos para una diversidad de virus mediante el ensayo de cultivo de células Vero. Mediante el empleo de esta técnica, hemos detectado nueve virus distintos de 4 familias taxonómicas de mosquitos recogidos en Connecticut.
Tags
Infectious VirusField-collected MosquitoesVero Cell Culture AssayWest Nile VirusDengue VirusChickungunya VirusSurveillance And Control ProgramsViral InfectionTrap LocationSpecies GroupingPooled SpecimensBuffered SalineMixer-millVero Cell CulturesCytopathic EffectDiagnostic AssaysVirus IsolationConnecticut USAHuman DiseaseNew Records
