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Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

DOI:
10.3791/2910-v

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16:13 min

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June 13, 2011

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, , ,
1Department of Neurology and Committee on Neurobiology,The University of Chicago Medical Center, 2Department of Neurology,The University of Chicago Medical Center

Chapters

  • 00:05Title
  • 02:13Materials Fabrication
  • 08:04Trans-Synaptically Induced Spreading Depression
  • 10:05Vital Imaging in Hippocampal Slice Cultures
  • 11:52RNA Extraction for Low-level Cytokine Signaling Detection
  • 12:47Proteomic Confirmations
  • 13:06Representative Results: Modeling Neural Immune Signaling of Episodic and Chronic Migraine
  • 15:07Conclusion

Summary

Automatic Translation

Automatic Translation

Migraine and its transformation to chronic migraine are immense healthcare burdens in need of improved treatment options. We seek to define how neural immune signaling modulates the susceptibility to migraine, modeled in vitro using spreading depression in hippocampal slice cultures, as a means to develop novel therapeutic targets.

Tags

Neural Immune SignalingEpisodic MigraineChronic MigraineSpreading DepressionIn Vitro ModelingTherapeutic TargetsMigraine AuraMigraine PainElectrical ActivityPain Perception StudiesTrigeminal Nociceptive PathwaysCellular MechanismsNeural Circuit MechanismsAcute SlicesHippocampal Slice Cultures

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