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August 14, 2011
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Test interazione proteina-proteina è indispensabile per la dissezione di funzionalità delle proteine. Qui, introdurre un<em> In vitro</em> Proteina-proteina saggio vincolante per sondare una membrana-proteina immobilizzato con una proteina solubile. Questa analisi fornisce un metodo affidabile per verificare l'interazione tra una proteina insolubile e una proteina in soluzione.
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Ueki, S., Lacroix, B., Citovsky, V. Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro. J. Vis. Exp. (54), e2961, doi:10.3791/2961 (2011).
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