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Förbereda E18 kortikala Råtta Nervceller för uppdelning i en mikroflödessystem enhet
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
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Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device
Please note that all translations are automatically generated.
Click here for the English version.
Förbereda E18 kortikala Råtta Nervceller för uppdelning i en mikroflödessystem enhet
DOI:
10.3791/305-v
•
12:00 min
•
October 01, 2007
•
Joseph Harris
,
Hyuna Lee
,
Christina Tu Tu
,
David Cribbs
,
Carl Cotman
,
Noo Li Jeon
1
Department of Biomedical Engineering
,
University of California, Irvine (UCI)
,
2
Stem Cell Research Center
,
University of California, Irvine (UCI)
,
3
Institute for Brain Aging and Dementia
,
University of California, Irvine (UCI)
Chapters
00:00
Title
00:03
Introduction
00:47
Preparing Tools and Reagents
01:46
Trypsinizing E18 Fetal Rat Cortex
04:14
Triturating E18 Fetal Rat Cortex
07:26
Loading Cortical Neurons into the Microfluidic Device
08:56
Visualizing Compartmentalized Cortical Neurons
09:37
Performing Axotomy for Axonal Regeneration Studies
11:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
I denna video visar vi utarbetandet av E18 kortikala Rat nervceller.
Tags
E18 Cortical Rat Neurons
Microfluidic Device
Preparation
Dissociation
Viability
Dissection Buffer
Trypsin
Incubation
DMEM
FBS
Centrifugation
Neural Basal Media (NBM)
Glutamax
Triturating
Cell Suspension
Nylon Ce
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