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重组DNA和蛋白质复合物，用原子力显微镜的可视化
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
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重组DNA和蛋白质复合物，用原子力显微镜的可视化

DOI:
10.3791/3061-v

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08:30 min

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July 18, 2011

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, ,
1College of Nursing, Interdisciplinary Life Sciences Research Laboratory,Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory,Seattle University

Chapters

  • 00:05Title
  • 01:12Preparing DNA and Protein Samples and Mounting AFM Probe
  • 02:43Preparing the AFM Prior to Sample Imaging
  • 04:33Imaging Mica Sample Surface and Biomolecules of Interest
  • 06:25Representative AFM Images
  • 07:48Conclusion

Summary

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是描述一个轻敲模式原子力显微镜（AFM）的可视化的质粒DNA，细胞质的蛋白质和DNA -蛋白质复合物的方法。该方法包括准备样品的原子力显微镜成像，生化操纵的替代方法。在近生理缓冲条件下观察DNA含有特定的蛋白相互作用的区域。

Tags

Atomic Force MicroscopyAFMVisualizationRecombinant DNAProtein ComplexesNano-scale ProbeElevation MeasurementMacromoleculesSubstrate SurfaceElectrostatic ForcesTopographical MapThree-dimensional ParticlesTapping Mode AFMBiomaterialsAqueous BuffersImmunoadsorbed ProteinsGlucocorticoid Receptor (GR)Buffered Solution

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