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Chapters
- 00:05Title
- 01:51Harvesting Embryos
- 03:10DiI Labeling of HH Stage 5 Embryos Using Magnetic Iron Particles
- 04:34Polystyrene Microsphere Labeling for OCT Imaging of HH11 – 12 Embryos
- 05:32Data Acquisition: Embryo Culture (General)
- 06:03Data Acquisition: Time-lapse Culture (Automated)
- 06:33Representative Results
- 07:41Conclusion
This article describes surface labeling and ex ovo tissue culture in the early chick embryo. Techniques amenable to time-lapse bright field, fluorescence, and optical coherence tomography imaging are presented. Tracking surface labels with high spatiotemporal resolution enables kinematic quantities such as morphogenetic strains (deformations) to be calculated in both two and three dimensions.
Tags
Early Chick EmbryoEmbryonic EpitheliaBendingTwistingFoldingStretchingPrimitive OrgansTracking Fiducial MarkersMorphogenetic QuantitiesGrowthContractionShearSurface Labeling TechniquesImaging ModalitiesFluorescent LabelsMagnetic Iron ParticlesPolystyrene MicrospheresContrast AgentsNon-invasive Optical Coherence Tomography (OCT) ImagingPhysical MechanismsHead Fold DevelopmentHeart DevelopmentBrain Development
