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Neural Tube stengning i Mouse Whole Embryo Kultur
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Neural Tube Closure in Mouse Whole Embryo Culture

Neural Tube stengning i Mouse Whole Embryo Kultur

DOI:
10.3791/3132-v

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07:37 min

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October 21, 2011

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1Department of Neurology/Neuroscience,Weill Cornell Medical College

Chapters

  • 00:05Title
  • 01:47Preparation of Culture Medium and Isolation of Uterus and Removal of Embryos
  • 03:59Setting up the Culture System
  • 05:20Evaluating Development after Whole Embryo Culture
  • 06:10Representative Embryo Cultures
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

En metode som åpner for direkte farmakologisk manipulasjon av mus embryo under neurulation som forbigår mors metabolisme er beskrevet. Teknikken kan tilpasses til å studere ulike aspekter ved neurulation ved å variere tidspunktet og farmakologiske agent.

Tags

Neural Tube ClosureMouse EmbryosEmbryo CultureGenetic Mouse ModelsMammalian Neural Tube ClosureMaternal MetabolismPharmacological ManipulationSmall Molecule DeliveryRecombinant Protein DeliverySiRNA DeliveryWhole Embryo CultureEnriched MediaRoller Incubator ApparatusNormal Neural Tube ClosureIn Vitro TechniquesNeurulationNeural Plate FormationCranial Fold ClosureCaudal Neuropore ClosureEmbryo Dissection

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