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Localizing Protein in 3D Neural Stem Cell Culture

Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology

Article DOI: 10.3791/2483 21:47 min December 19th, 2010
December 19th, 2010



Here, we describe how to produce, expand, and immunolabel postnatal hippocampal neural progenitor cells (NPCs) in three-dimensional (3D) culture. Next, using hybrid visualization technologies, we demonstrate how digital images of immunolabelled cryosections can be used to reconstruct and map the spatial position of immunopositive cells throughout the entire 3D neurosphere.


Protein Localization 3D Neural Stem Cell Culture Visualization Methodology Neurospheres Developmental Lineages Immunocytochemistry Target Proteins NPC Fate Cellular Positioning Cryosections Confocal Reconstructions
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