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Localizing Protein in 3D Neural Stem Cell Culture

Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology

Article DOI: 10.3791/2483
December 19th, 2010


Summary December 19th, 2010

Here, we describe how to produce, expand, and immunolabel postnatal hippocampal neural progenitor cells (NPCs) in three-dimensional (3D) culture. Next, using hybrid visualization technologies, we demonstrate how digital images of immunolabelled cryosections can be used to reconstruct and map the spatial position of immunopositive cells throughout the entire 3D neurosphere.

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