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Chapters
- 00:05Title
- 01:47Preparation of Culture Medium and Isolation of Uterus and Removal of Embryos
- 03:59Setting up the Culture System
- 05:20Evaluating Development after Whole Embryo Culture
- 06:10Representative Embryo Cultures
- 07:08Conclusion
Un método que permite la manipulación directa farmacológico de embriones de ratón durante la neurulación que no pasa por el metabolismo materno se describe. La técnica se puede adaptar a estudiar diferentes aspectos de la neurulación variando el punto en el tiempo y el agente farmacológico.
Tags
Neural Tube ClosureMouse EmbryosEmbryo CultureGenetic Mouse ModelsMammalian Neural Tube ClosureMaternal MetabolismPharmacological ManipulationSmall Molecule DeliveryRecombinant Protein DeliverySiRNA DeliveryWhole Embryo CultureEnriched MediaRoller Incubator ApparatusNormal Neural Tube ClosureIn Vitro TechniquesNeurulationNeural Plate FormationCranial Fold ClosureCaudal Neuropore ClosureEmbryo Dissection
