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Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
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Chapters
- 00:05Title
- 02:07Mouse Infection with γHV68
- 03:16Preparation of Cell Lysate from Lung Tissue
- 04:32Plaque Assay Procedure
- 06:41Determine γHV68 Multi-step Growth Kinetics in Mouse Embryonic Fibroblasts
- 08:15Molecular Dissection of γHV68 Lytic Replication in Mouse Embryonic Fibroblasts
- 09:28Representative Results
- 10:58Conclusion
We describe a protocol to identify key roles of host signaling molecules in lytic replication of a model herpesvirus, gamma herpesvirus 68 (γHV68). Utilizing genetically modified mouse strains and embryonic fibroblasts for γHV68 lytic replication, the protocol permits both phenotypic characterization and molecular interrogation of virus-host interactions in viral lytic replication.
Tags
Host-virus InteractionLytic ReplicationModel HerpesvirusViral InfectionInnate Immune Signaling PathwaysAntiviral Cytokine ProductionViral EvasionSignaling Pathways ManipulationTherapeutic StrategiesMurine γHV68Kaposi’s Sarcoma-associated HerpesvirusEpsten-Barr VirusSmall Animal ModelPhenotypic CharacterizationMolecular DissectionHost Signaling ComponentsGenetically Modified Mouse StrainsAcute InfectionMouse Embryonic Fibroblasts (MEFs)Virological AssaysMolecular Biology Assays
