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从人类乳腺成纤维细胞癌相关的成纤维细胞的实验发电(CAFS)
JoVE Journal
Medicine
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JoVE Journal Medicine
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
DOI:

15:43 min

October 25, 2011

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Chapters

  • 00:05Title
  • 02:46Isolation of Primary Cultured Human Normal Mammary Fibroblasts
  • 04:43Generation of GFP-labeled, Puromycin-Resistant and Immortalized Human Normal Mammary Fibroblasts
  • 07:35Co-injection of Human Mammary Fibroblasts with Breast Carcinoma Cells into an Immunodeficient Mouse
  • 08:58Extraction of CAFs or their Corresponding Control Fibroblasts from the Tumor or Fibroblast Xenografts, Respectively
  • 13:31Representative Images of Experimentally Generated CAFs
  • 15:18Conclusion

Summary

Automatic Translation

癌相关成纤维细胞(CAFS)在肌纤维母细胞肿瘤间质内的丰富,推动肿瘤进展发挥了重要作用。从人类乳腺成纤维细胞的实验产生CAFS我们开发了一个coimplantation肿瘤xengraft模型。协议描述了如何建立非洲足联的肌纤维母细胞的获取能力,以促进tumourigenesis。

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