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Experimentell generationens Cancer Associated-fibroblasts (CAFS) från Human bröst fibroblasts
JoVE Journal
Medicine
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JoVE Journal Medicine
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
DOI:

15:43 min

October 25, 2011

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Chapters

  • 00:05Title
  • 02:46Isolation of Primary Cultured Human Normal Mammary Fibroblasts
  • 04:43Generation of GFP-labeled, Puromycin-Resistant and Immortalized Human Normal Mammary Fibroblasts
  • 07:35Co-injection of Human Mammary Fibroblasts with Breast Carcinoma Cells into an Immunodeficient Mouse
  • 08:58Extraction of CAFs or their Corresponding Control Fibroblasts from the Tumor or Fibroblast Xenografts, Respectively
  • 13:31Representative Images of Experimentally Generated CAFs
  • 15:18Conclusion

Summary

Automatic Translation

Cancer-associerade fibroblaster (CAFS) med rik myofibroblaster vistas i tumören glatta spela en viktig roll i att driva tumörtillväxt. Vi utvecklade en coimplantation modell tumör xengraft för experimentellt generera CAFS från mänsklig bröst fibroblaster. Protokollet beskriver hur man kan upprätta CAF myofibroblaster att förvärva en förmåga att främja tumourigenesis.

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