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Journal
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Immunology and Infection
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成像白细胞粘附到血管内皮细胞在高腔内压力
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure
Please note that all translations are automatically generated.
Click here for the English version.
成像白细胞粘附到血管内皮细胞在高腔内压力
DOI:
10.3791/3221-v
•
06:20 min
•
August 23, 2011
•
Danielle L. Michell
,
Karen L. Andrews
,
Kevin J. Woollard
,
Jaye P.F. Chin-Dusting
1
Vascular Pharmacology Laboratory, Baker IDI Heart and Diabetes Institute
,
Monash University
Chapters
00:05
Title
01:11
Mounting the Vessel
02:13
Pressurizing the Vessel
04:19
Perfusion with While Blood
05:18
Representative Leukocyte Adhesion Results
06:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这是一个可视化白细胞粘附到内皮收获压力容器的方法。该技术使研究不同的腔内压力高达200毫米汞柱,从而模拟高血压的病理生理条件下剪切流动的血管粘连。
Tags
Leukocyte Adhesion
Vascular Endothelium
High Intraluminal Pressure
Hypertension
Endothelial Dysfunction
Inflammation
Atherosclerosis
Chronic Kidney Disease
Stroke
Heart Failure
Adhesion Cascade
Rolling
Tethering
Adherence
Transmigration
Adhesion Molecules
VCAM-1
ICAM-1
E-selectin
Cytokines
Chemokines
Reactive Oxygen Species
Static Adhesion Assays
Cell-to-cell Adhesion
Cell Adhesion Molecules
In Vitro Methods
Pressure On Endothelium
Monocyte Adhesion
Inflammatory Markers
Physiological Flow Conditions
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