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高效的推导人类心脏的前体和从多能干的小分子诱导人类胚胎干细胞的心肌细胞
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
Please note that all translations are automatically generated.
Click here for the English version.
高效的推导人类心脏的前体和从多能干的小分子诱导人类胚胎干细胞的心肌细胞
DOI:
10.3791/3274-v
•
10:46 min
•
November 03, 2011
•
Xuejun H. Parsons
2
,
Yang D. Teng
4
,
James F. Parsons
2
,
Evan Y. Snyder
2,5
,
David B. Smotrich
2,6
,
Dennis A. Moore
2
1
San Diego Regenerative Medicine Institute
,
2
Xcelthera
,
3
Department of Neurosurgery
,
Harvard Medical School
,
4
Division of SCI Research
,
VA Boston Healthcare System
,
5
Program in Stem Cell & Regenerative Biology
,
Sanford-Burnham Medical Research Institute
,
6
La Jolla IVF
Chapters
00:05
Title
01:22
Media Preparation and Plate Coating
02:30
Passing and Seeding Undifferentiated Human ES Cells
04:27
Cardiac Induction in Adhesive Culture with Nicotinamide
05:37
Differentiation in Suspension Culture and Maturation to Beating Cardiomyocyte Phenotype after Attachment
08:05
Direct Cardiomyocyte Differentiation of Feeder- and Serum-Free Pluripotent Humand ES Cells with Nicotinamide Induction
10:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们已经建立了诱导多能干人类胚胎干细胞与小分子物质,使推导一个人类心脏的祖细胞和心血管修复功能心肌的大量供应定义的条件下保持直接从cardioblasts协议。
Tags
Human Cardiac Precursors
Cardiomyocytes
Pluripotent Human Embryonic Stem Cells
Small Molecule Induction
Regenerating Human Myocardium
Cell-based Transplantation
Stem/progenitor Cells
Bone Marrow
Cord Blood
Contractile Heart Muscle Cells
Adult Stem Cell Therapy
Genetically Stable Human Embryonic Stem Cells
Unlimited Expansion Ability
Unrestricted Plasticity
Large Supplies Of Human Somatic Cells
Cardiovascular Disease
Donor Organs
HESC-based Therapies
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