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Profilage Single Cell transcriptionnelle des cardiomyocytes de souris adultes

Article DOI: 10.3791/3302
December 28th, 2011 •
1, 2, 1
1Buck Institute for Research on Aging, 2Department of Physiology & Biophysics, University of Washington

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December 28th, 2011

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Click here for the English version.

Simple cellule de profilage de l'expression génique permet l'analyse détaillée d'expression des cellules individuelles. Nous décrivons des méthodes pour l'isolement des cardiomyocytes, et la préparation des lysats résultant soit pour tout ou biopuces transcriptome qPCR d'objectifs spécifiques.

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