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Biology
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成年小鼠心肌细胞的单细胞转录谱
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
Please note that all translations are automatically generated.
Click here for the English version.
成年小鼠心肌细胞的单细胞转录谱
DOI:
10.3791/3302-v
•
08:23 min
•
December 28, 2011
•
James M. Flynn
,
Luis F. Santana
,
Simon Melov
1
Buck Institute for Research on Aging
,
2
Department of Physiology & Biophysics
,
University of Washington
Chapters
00:05
Title
01:15
Isolating Cardiomyocyte
03:37
Isolating Single Cells
04:08
Single Cell qPCR
05:26
Whole Transcriptome Amplification and Microarray of Single Cells
07:00
Quality Control Results
07:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
单细胞的表达谱可以详细的单个细胞的基因表达分析。我们描述为心肌细胞隔离的方法,并编制要么全转录组微阵列或qPCR的具体目标产生的裂解物。
Tags
Single Cell
Transcriptional Profiling
Adult Mouse
Cardiomyocytes
Gene Expression
Homogenates
Stochastic Variation
Pure Cardiomyocyte Populations
Collagenase Perfusion
Microarrays
Whole Transcriptome
QPCR
Nanofluidic Arrays
Gene Expression Profiles
Evaluation Metrics
Variance Between Cells
Robust Amplification
Double Stranded CDNA
NimbleGen Arrays
Reverse Transcriptase – Specific Target Amplification (RT-STA)
Nanofluidic PCR
Variability Of Expression
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