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Journal
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Bioengineering
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平行板流动腔和评估内皮祖细胞层流剪应力下连续流电路
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
Please note that all translations are automatically generated.
Click here for the English version.
平行板流动腔和评估内皮祖细胞层流剪应力下连续流电路
DOI:
10.3791/3349-v
•
12:43 min
•
January 17, 2012
•
Whitney O. Lane
,
Alexandra E. Jantzen
,
Tim A. Carlon
,
Ryan M. Jamiolkowski
,
Justin E. Grenet
,
Melissa M. Ley
,
Justin M. Haseltine
,
Lauren J. Galinat
,
Fu-Hsiung Lin
,
Jason D. Allen
,
George A. Truskey
,
Hardean E. Achneck
1
Department of Surgery
,
Duke University Medical Center
,
2
Department of Biomedical Engineering
,
Duke University
,
3
School of Medicine
,
University of Pennsylvania
,
4
Department of Medicine, Division of Cardiology
,
Duke University Medical Center
Chapters
00:05
Title
02:20
Flow Circuit Assembly
05:30
Flow Chamber Assembly
08:17
Perfusate-sample Collection
09:08
Evaluation of Endothelial Progenitor Cells under Laminar Flow Shear Stress
11:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们所描述的主题贴壁的细胞在无菌的连续流电路层流剪应力的方法。细胞的黏附,形态可通过透明腔研究,从代谢物的分析和对未来的实验或文化的剪切曝光后的干细胞的电路样本。
Tags
Parallel-plate Flow Chamber
Continuous Flow Circuit
Endothelial Progenitor Cells
Laminar Flow Shear Stress
Cell Adhesion
Cell Morphology
Human Umbilical Cord Blood-derived Endothelial Progenitor Cells
Porcine EPCs
Smooth Muscle Cells
Fibroblasts
Microfluidic Chambers
Sterilization
Cell Recovery
DNA Extraction
RNA Extraction
Immunohistochemistry
Scanning Electron Microscopy
Shear Stress Adjustment
Perfusate Flow Rate
Fluid Viscosity
Channel Height And Width
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