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由一个小型的双特异性亲和标签的推动正交蛋白质纯化
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
DOI:

10:32 min

January 16, 2012

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Chapters

  • 00:05Title
  • 02:05Cloning of the Target Gene/ABDz1-tagged Fusion Construct
  • 04:02Protein Expression
  • 04:49Orthogonal Affinity Purification
  • 08:04Results: SDS-PAGE Analysis of Purified Protein Products
  • 09:45Conclusion

Summary

Automatic Translation

已开发一种新的和高效两步亲和层析协议,并进行了详细描述。该方法是基于一个小净化标签有两个固有的亲和力,适用于广泛的目标蛋白质具有不同的属性。

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