JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
由一个小型的双特异性亲和标签的推动正交蛋白质纯化
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
Please note that all translations are automatically generated.
Click here for the English version.
由一个小型的双特异性亲和标签的推动正交蛋白质纯化
DOI:
10.3791/3370-v
•
10:32 min
•
January 16, 2012
•
Johan Nilvebrant
,
Tove Alm
,
Sophia Hober
1
School of Biotechnology, Department of Proteomics
,
Royal Institute of Technology
Chapters
00:05
Title
02:05
Cloning of the Target Gene/ABDz1-tagged Fusion Construct
04:02
Protein Expression
04:49
Orthogonal Affinity Purification
08:04
Results: SDS-PAGE Analysis of Purified Protein Products
09:45
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
已开发一种新的和高效两步亲和层析协议,并进行了详细描述。该方法是基于一个小净化标签有两个固有的亲和力,适用于广泛的目标蛋白质具有不同的属性。
Tags
Orthogonal Protein Purification
Small Bispecific Affinity Tag
Recombinant Proteins
Purification Protocols
High-throughput Methods
Purification Tags
Hexa-histidine Tag
Affinity Chromatography
Bispecific Purification Tag
Albumin-binding Domain
Streptococcal Protein G
Human Serum Albumin (HSA)
Phage Display Technology
Dimeric Z-domain
Staphylococcal Protein A
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
无有机溶剂和醋酸,蛋白质与考马斯亮蓝G - 250凝胶染色
由不稳定的多蛋白质复合物的分离
在体内
控制细胞的交联和免疫磁性亲和层析
Hsp104纯化,蛋白质Disaggregase
鉴定蛋白质相互作用的合作伙伴使用串联亲和纯化
蛋白复合物的鉴定<em>大肠杆菌</em使用顺序肽亲和纯化技术相结合串联质谱法
高通量的亲和标签的重组蛋白的纯化
GST-他的净化:一个两步亲和纯化协议屈服全长纯化的蛋白质
蛋白质纯化方法测定结合亲和力微尺度热泳
一个协议,用于噬菌体展示和亲和选择以重组蛋白诱饵
鉴定蛋白质 - 蛋白质相互作用的<em>果蝇</em>成人头由串联亲和纯化(TAP)
Read Article