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Journal
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Bioengineering
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伸展的DNA短序列恒力轴向光镊
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
Please note that all translations are automatically generated.
Click here for the English version.
伸展的DNA短序列恒力轴向光镊
DOI:
10.3791/3405-v
•
08:48 min
•
October 13, 2011
•
Krishnan Raghunathan
,
Joshua N. Milstein
,
Jens -Christian Meiners
1
LSA Biophysics
,
University of Michigan
,
2
LSA Biophysics, Department of Physics
,
University of Michigan
Chapters
00:05
Title
01:07
Tweezer Setup
02:45
Calibrating Apparent Size of the Bead to Axial Position
04:16
Mapping the Optical Potential of the Manipulation Beam
05:10
Stretching the DNA Sample
08:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们说明了使用一个恒定的力的轴向光镊探索短的DNA分子的力学性能。轴向拉伸DNA,我们尽量减少在传统的横向操作所产生的立体障碍和文物,使我们能够研究DNA分子的短〜100 nm之间。
Tags
Stretching
Short Sequences
DNA
Constant Force
Axial Optical Tweezers
Single-molecule Techniques
Experimental Difficulties
Biological Events
Histone Binding
Protein-mediated Looping
Mechanical Properties
Packaging Of DNA
Compact Nucleosomes
Chromatin Fibers
Practical Guide
Optical Tweezing Technique
Contour Lengths
Submicron Length DNA
Bead
Coverslip
Axial Position
Steric Effects
Microspheres
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