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Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers
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Bioengineering
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JoVE Journal Bioengineering
Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

DOI:
10.3791/3520-v

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10:08 min

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July 25, 2012

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, ,
1Department of Chemical Engineering,Stanford University

Chapters

  • 00:05Title
  • 01:40Flow Cell Preparation
  • 02:52Magnetic Tweezers Set-up and Calibration
  • 05:55Collagen Peptide Attachment to Flow Cells
  • 07:00Force Proteolysis Assay
  • 07:56Results: The Effect of Force on Enzymatic Proteolysis
  • 08:46Conclusion

Summary

Automatic Translation

Automatic Translation

In this article we describe the use of magnetic tweezers to study the effect of force on enzymatic proteolysis at the single molecule level in a highly parallelizable manner.

Tags

MultiplexedSingle-moleculeForceProteolysisMeasurementsMagnetic TweezersGenerationDetectionMechanical ForcesCell PhysiologyCancer MetastasisAtherogenesisWound HealingExtracellular Matrix (ECM)Enzymatically RemodelForces On ECMBiological ImportanceMedical ImportanceSingle Molecule TechniquesOptical TrappingAtomic Force MicroscopyMagnetic Tweezers (MT)Low CostHigh ThroughputPN (piconewton)Millisecond Temporal ResolutionEnzyme MechanismHighly Parallelizable AssayTrimeric Collagen PeptideMatrix Metalloproteinase 1 (MMP-1)Substrates And Proteases

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