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Biology
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哺乳动物细胞培养,使用一种新的多层瓶中的比例增加
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
Please note that all translations are automatically generated.
Click here for the English version.
哺乳动物细胞培养,使用一种新的多层瓶中的比例增加
DOI:
10.3791/3418-v
•
08:39 min
•
December 05, 2011
•
Elizabeth J. Abraham
,
Katie A. Slater
,
Suparna Sanyal
,
Ken Linehan
,
Paula M. Flaherty
,
Susan Qian
1
BD Biosciences
Chapters
00:05
Title
01:00
Design and Preparation of Falcon Multi-Flasks and Addition of Cell Suspensions
02:19
Mixing of Cells: Equilibration and Partitioning of Fluids
03:23
Transportation and Placement of the Multi-Flask
04:12
Media Exchange and Cell Harvesting
05:40
Recommended Working Volume in Falcon Multi-Flasks
06:10
Homogenous Cell-Population Generation
08:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
细胞发挥在研究的工具和越来越大的作用,并发现和开发新疗法。有了这个我们需要更有效率和更有效的方式,为种植和收获附件依赖细胞的细胞数量增加的需要。一个正确的功能的多层瓶中可以达到这个目的。
Tags
Mammalian Cell Culture
Scale-up
Multilayered Flask
Cell-based Applications
T-flasks
Large Numbers Of Cells
Multi-layered Cell Culture Vessel
BD Multi-Flask
Mix/equilibration Port
Uniform Distribution
Pipette Access
Efficient Recovery
Risk Of Contamination
Residual Liquid Retention
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