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生长实验,以评估在酵母中的多聚谷氨酰胺毒性
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Biology
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Biology
Growth Assays to Assess Polyglutamine Toxicity in Yeast
Please note that all translations are automatically generated.
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生长实验,以评估在酵母中的多聚谷氨酰胺毒性
DOI:
10.3791/3461-v
•
09:06 min
•
March 05, 2012
•
Martin L. Duennwald
1
Boston Biomedical Research Institute
Chapters
00:05
Title
01:25
Experimental Considerations and Set-up of Yeast Cells Expressing Toxic PolyQ-expansion Proteins
03:26
Plating Assay
04:30
Spotting Assay
05:25
Liquid Growth Assay
07:08
Representative Growth Assay Results
08:30
Conclusion
Summary
Automatic Translation
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Automatic Translation
这手稿描述了三个评估毒性的多聚谷氨酰胺(polyQ蛋白)扩张蛋白在酵母的补充协议<em>酿酒酵母</em>。这些协议可以很容易地进行修改,以监察在酵母中错误折叠的蛋白质的毒性。
Tags
Growth Assays
Assess
Polyglutamine Toxicity
Yeast
Protein Misfolding
Neurodegenerative Diseases
Huntington’s Disease
Cellular Toxicity
PolyQ-expansions
Spinocerebellar Ataxias
Kennedy’s Disease
Model Organism Yeast
Cellular Pathways
Experimental Systems
HD Patients
Basic Mechanisms
Growth Defects
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