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Neuroscience
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从E13小鼠胶质限制前体的推导
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Derivation of Glial Restricted Precursors from E13 mice
Please note that all translations are automatically generated.
Click here for the English version.
从E13小鼠胶质限制前体的推导
DOI:
10.3791/3462-v
•
08:56 min
•
June 20, 2012
•
André W. Phillips
2
,
Sina Falahati
2
,
Roshi DeSilva
3
,
Irina Shats
,
Joel Marx
,
Edwin Arauz
,
Douglas A. Kerr
,
Jeffrey D. Rothstein
5
,
Michael V. Johnston
2,6
,
Ali Fatemi
2,6
1
Hugo W. Moser Research Institute at Kennedy Krieger
,
Johns Hopkins University
,
2
Department of Neurology
,
Johns Hopkins School of Medicine
,
3
University of Maryland
,
4
Experimental Neurology
,
Biogen Idec
,
5
The Brain Science Institute
,
Johns Hopkins School of Medicine
,
6
Department of Pediatrics
,
Johns Hopkins School of Medicine
Chapters
00:05
Title
01:05
Tissue Derivation
03:48
Cell Dissociation and Culture Establishment
05:19
Immunopanning the GRP Population
06:27
Freezing and Storage of Derived Cells
07:24
Purified GRP Cells
08:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议概述从胎儿的脊髓神经胶质细胞的限制性前体推导,并保持在体外移植或oligodendrocytic谱系研究。
Tags
Glial Restricted Precursor
GRP Cells
White Matter Diseases
Periventricular Leukomalacia
PVL
Oligodendrocyte Progenitor Cells
OPC
Immature Oligodendrocytes
PreOL
Hypoxia-ischemia
Oxidative Stress
Excitotoxicity
Glutamate Excitotoxicity
Oligodendrocyte Progenitors
Transplantation Experiments
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