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Journal
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Immunology and Infection
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确定临床抗体样品的吞噬活性
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Determining the Phagocytic Activity of Clinical Antibody Samples
Please note that all translations are automatically generated.
Click here for the English version.
确定临床抗体样品的吞噬活性
DOI:
10.3791/3588-v
•
09:05 min
•
November 30, 2011
•
Elizabeth G. McAndrew
,
Anne-Sophie Dugast
,
Anna F. Licht
,
Justin R. Eusebio
,
Galit Alter
,
Margaret E. Ackerman
1
Massachusetts General Hospital
,
Ragon Institute of MGH, MIT, and Harvard
,
2
Thayer School of Engineering
,
Dartmouth College
Chapters
00:05
Title
02:01
Preparing Biotinylated Antigen
02:58
Preparing Antigen Saturated Beads
04:08
Preparing Antibody Samples and Plating the Experiment
05:44
Flow Cytometric Analysis
06:29
Antigen-saturated Beads and Phagocytosed in an Antibody-dependent Manner
08:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们目前的高通量流式细胞仪检测,以确定从临床标本中的抗原特异性抗体的吞噬活性,利用荧光抗原包被的磁珠和单核细胞表达多个Fc受体提供一个标准化的受体的使用和吞噬活性测定重现时尚任何利益抗原。
Tags
Phagocytic Activity
Clinical Antibody Samples
Fc Receptors
Clearance
Pathology Of Disease
Variable Domains
Innate Immune Cells
Recombinant Monoclonal Antibody Therapy
Natural Infection
Vaccination
IgG Subclass
Glycosylation
Functional Differences
Antigen-specific Antibodies
Disease State
Susceptibility To Infection
Progression
Clinical Outcome
Effector Function
Enhance Infection
Fc Receptor-driven Phagocytosis
Immune Complexes
Th1/Th2 Polarization Assay
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