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Chapters
- 00:05Title
- 01:28Morpholino Preparation and Removing the Outer Corneal Layer
- 02:32Corneal Incision and Morpholino Injection
- 03:31Electroporation
- 04:55Representative Morpholino Electroporation Results
- 06:14Conclusion
En metode til betinget Knockdown et mål protein udtryk i den voksne zebrafisk nethinden er beskrevet, hvilket indebærer intravitrealt injektion antisense morpholinos og electroporating dem ind i nethinden. Den resulterende protein er slået ned i flere dage, hvilket giver mulighed for test af protein rolle i regenerere eller intakte nethinden.
Tags
In Vivo ElectroporationMorpholinosAdult Zebrafish RetinaInherited Eye DiseasesProgressive BlindnessRetinal NeuronsRegenerationRetinal Damage ModelsPhotoreceptorsInner Retinal NeuronsMüller GliaNeuronal ProgenitorsRetinal LayerDifferentiationCellular ResponsesMRNA Microarray AnalysisOcular DevelopmentCandidate Gene/protein
