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JoVE Journal
Biology

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In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
 
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In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina

Article doi: 10.3791/3603
December 27th, 2011

Summary December 27th, 2011

Please note that all translations are automatically generated.

Click here for the English version.

A method to conditionally knockdown a target protein’s expression in the adult zebrafish retina is described, which involves intravitreally injecting antisense morpholinos and electroporating them into the retina. The resulting protein is knocked down for several days, which allows testing the protein’s role in the regenerating or intact retina.

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