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体外器官角膜模型急性上皮疱疹,单纯疱疹病毒I型感染
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection
DOI:

07:55 min

November 03, 2012

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Chapters

  • 00:00Title
  • 00:47Introduction
  • 02:08Materials
  • 02:36Excision of the Corneoscleral Button
  • 03:29Introduction of Cornea into Organotypic Culture
  • 04:22Infection with HSV-1 and Treatment with Experimental Drugs
  • 05:25Medium for Plaque Assays
  • 05:45DNA, RNA, Protein, or Cells from Corneal Epithelium
  • 06:48Tissue Samples for Immunohistochemistry or Immunofluorescence
  • 07:23Conclusion

Summary

Automatic Translation

在这个视频文章中,我们描述了使用一个新的<em>体外</em>模型急性疱疹单纯病毒I型角膜上皮感染。

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