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1Department of Microbiology and Parasitology, College of Basic Medical Sciences,China Medical University , 2Department of Obstetrics and Gynecology, College of Medicine,University of Arkansas for Medical Sciences , 3Department of Pathology, College of Medicine,University of Arkansas for Medical Sciences
Chapters
- 00:05Title
- 01:59Limiting Dilution of Epitope-specific T Cells
- 03:45Identifying Epitope-specific T-cell Clones: High-throughput Screening ELISPOT Assay
- 07:10Confirm Epitope-specific Nature of the T-cell Clones
- 07:30Is the Epitope Endogenously Processed?
- 08:09Characterizing the Minimal and Optimal Sequence of the T-cell Epitope
- 08:39Identify the Restriction Element Using the ELISPOT Assay
- 09:09Crossreactivity of the Epitope-specific T-cell Clones to Homologous Amino Acid Sequences from Other High-risk HPV Types
- 09:32A Clinical Case
- 13:08Conclusion
Characterizing T-cell epitopes of pathogens that cause localized infections such as human papillomavirus is a challenge because of limited number of T cells in circulation. A method is described in which rare T cells were isolated and were characterized starting with a very small number of cells.
Tags
Interferon-gammaEnzyme-linked Immunospot AssayT-cell EpitopesHuman Papillomavirus (HPV)ProtocolIsolating And Characterizing Rare T CellsHPV ProteinsPeptide-specific T CellsLoop Electrical Excision ProcedureHigh-grade Squamous Intraepithelial LesionHuman Papillomavirus Type 16 (HPV 16)E6 And E7 ProteinsIFN-g Enzyme-linked Immunospot (ELISPOT) AssaySynthetic PeptidesEpitope Specific T CellsCD8 T-cell Isolation KitMiltenyi Biotec
