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表面等离子体共振检测到宿主细胞质的毒素易位
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
DOI:

10:41 min

January 03, 2012

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Chapters

  • 00:05Title
  • 01:31Cell Intoxication and Permeabilization
  • 03:34Sample and Standard Toxin Preparation
  • 06:11Surface Plasmon Resonance
  • 07:08Representative Surface Plasmon Resonance Traces and Analysis
  • 10:18Conclusion

Summary

Automatic Translation

在这份报告中,我们描述了表面等离子体共振是如何用来检测输入到宿主细胞质中的毒素。胞浆内毒素量,这个高度敏感的方法可以提供定量数据,并可以应用到一系列的毒素。

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