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Immunology and Infection
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表面等离子体共振检测到宿主细胞质的毒素易位
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Immunology and Infection
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Please note that all translations are automatically generated.
Click here for the English version.
表面等离子体共振检测到宿主细胞质的毒素易位
DOI:
10.3791/3686-v
•
10:41 min
•
January 03, 2012
•
Michael Taylor
,
Tuhina Banerjee
,
Neyda VanBennekom
,
Ken Teter
1
Department of Molecular Biology and Microbiology
,
University of Central Florida
Chapters
00:05
Title
01:31
Cell Intoxication and Permeabilization
03:34
Sample and Standard Toxin Preparation
06:11
Surface Plasmon Resonance
07:08
Representative Surface Plasmon Resonance Traces and Analysis
10:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这份报告中,我们描述了表面等离子体共振是如何用来检测输入到宿主细胞质中的毒素。胞浆内毒素量,这个高度敏感的方法可以提供定量数据,并可以应用到一系列的毒素。
Tags
Toxin Translocation
Surface Plasmon Resonance
AB Toxins
Cell-binding B Subunit
Vesicle Carriers
Endoplasmic Reticulum (ER)
Catalytic A Chain
Protein-conducting Channel
Cytosolic Target
Toxin Trafficking
Lysosomes
Golgi Apparatus
Subcellular Fractionation Protocol
Digitonin Permeabilization
Anti-toxin A Chain Antibody
Detection Method
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