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Lateral Diffusion og exocytose af membranproteiner i dyrkede neuroner vurderes ved hjælp af Fluorescens Recovery og Fluorescens-tab fotoblegning
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

Lateral Diffusion og exocytose af membranproteiner i dyrkede neuroner vurderes ved hjælp af Fluorescens Recovery og Fluorescens-tab fotoblegning

DOI:
10.3791/3747-v

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11:58 min

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February 29, 2012

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, , ,
1MRC Centre for Synaptic Plasticity,University of Bristol

Chapters

  • 00:05Title
  • 01:33Cell Culture, Viral Transduction, and Protein Expression
  • 02:16FRAP-FLIP Live Cell Imaging: Equipment Set-up and Image Capture Parameters
  • 05:48FRAP-FLIP Live Cell Imaging: Image Acquisition
  • 07:09Data Analysis
  • 09:25Representative FRAP-FLIP Results
  • 11:34Conclusion

Summary

Automatic Translation

Automatic Translation

Denne rapport beskriver anvendelsen af ​​levende celler billeddannelse og photobleach teknikker til bestemmelse af overfladen ekspression transportveje og handel kinetik eksogent udtrykt, pH-følsom GFP-mærkede proteiner til plasmamembranen af ​​neuroner.

Tags

Lateral DiffusionExocytosisMembrane ProteinsCultured NeuronsFluorescence RecoveryFluorescence-loss PhotobleachingTraffickingReceptorsIon ChannelsSynaptic ProteinsSuper-ecliptic PHluorinLive Cell ImagingPlasma Membrane ProteinsProtonationPhoton AbsorptionFluorescence EmissionIntracellular Trafficking EventsLow PH CompartmentsNeutral PH Extracellular EnvironmentPhotodamagePhotobleachingFRAP (fluorescence Recovery After Photobleaching)Protein Dynamics

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