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横向扩散和培养的神经元膜蛋白的胞吐评估使用荧光恢复和荧光损失漂白
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
Please note that all translations are automatically generated.
Click here for the English version.
横向扩散和培养的神经元膜蛋白的胞吐评估使用荧光恢复和荧光损失漂白
DOI:
10.3791/3747-v
•
11:58 min
•
February 29, 2012
•
Keri L. Hildick
,
Inmaculada M. González-González
,
Frédéric Jaskolski
,
Jeremy. M. Henley
1
MRC Centre for Synaptic Plasticity
,
University of Bristol
Chapters
00:05
Title
01:33
Cell Culture, Viral Transduction, and Protein Expression
02:16
FRAP-FLIP Live Cell Imaging: Equipment Set-up and Image Capture Parameters
05:48
FRAP-FLIP Live Cell Imaging: Image Acquisition
07:09
Data Analysis
09:25
Representative FRAP-FLIP Results
11:34
Conclusion
Summary
Automatic Translation
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这份报告介绍了利用活细胞成像和光漂白技术,以确定表面的表达,运输途径和贩运外生表示,pH敏感的绿色荧光蛋白标记的蛋白质在神经元细胞膜动力学。
Tags
Lateral Diffusion
Exocytosis
Membrane Proteins
Cultured Neurons
Fluorescence Recovery
Fluorescence-loss Photobleaching
Trafficking
Receptors
Ion Channels
Synaptic Proteins
Super-ecliptic PHluorin
Live Cell Imaging
Plasma Membrane Proteins
Protonation
Photon Absorption
Fluorescence Emission
Intracellular Trafficking Events
Low PH Compartments
Neutral PH Extracellular Environment
Photodamage
Photobleaching
FRAP (fluorescence Recovery After Photobleaching)
Protein Dynamics
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