JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
培養神経細胞における膜タンパク質の側方拡散とエキソサイトーシスは、蛍光回復と蛍光損失光退色を用いて評価
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
Please note that all translations are automatically generated.
Click here for the English version.
培養神経細胞における膜タンパク質の側方拡散とエキソサイトーシスは、蛍光回復と蛍光損失光退色を用いて評価
DOI:
10.3791/3747-v
•
11:58 min
•
February 29, 2012
•
Keri L. Hildick
,
Inmaculada M. González-González
,
Frédéric Jaskolski
,
Jeremy. M. Henley
1
MRC Centre for Synaptic Plasticity
,
University of Bristol
Chapters
00:05
Title
01:33
Cell Culture, Viral Transduction, and Protein Expression
02:16
FRAP-FLIP Live Cell Imaging: Equipment Set-up and Image Capture Parameters
05:48
FRAP-FLIP Live Cell Imaging: Image Acquisition
07:09
Data Analysis
09:25
Representative FRAP-FLIP Results
11:34
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
このレポートでは、ニューロンの細胞膜に発現する外因性、pH感受性GFPタグ融合タンパク質の表面発現、輸送経路や人身売買の速度を決定するための生細胞イメージングと光退色技術の使用について説明します。
Tags
Lateral Diffusion
Exocytosis
Membrane Proteins
Cultured Neurons
Fluorescence Recovery
Fluorescence-loss Photobleaching
Trafficking
Receptors
Ion Channels
Synaptic Proteins
Super-ecliptic PHluorin
Live Cell Imaging
Plasma Membrane Proteins
Protonation
Photon Absorption
Fluorescence Emission
Intracellular Trafficking Events
Low PH Compartments
Neutral PH Extracellular Environment
Photodamage
Photobleaching
FRAP (fluorescence Recovery After Photobleaching)
Protein Dynamics
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
培養海馬ニューロンの樹状突起棘の蛍光タグ融合タンパク質の光退色後蛍光回復(FRAP)
ライブラット皮質ニューロンのミトコンドリア膜電位と活性酸素種の定量
培養中枢神経系ニューロンの樹状突起スパイン形態の解析
FM染料を用いて培養小脳顆粒神経細胞のシナプス小胞プールの補充の定量分析
培養交感神経ニューロンの樹状成長を誘導する
イメージング初代培養マウス神経細胞における形質膜に受容体の挿入をpHluorinタグ付き
ライブ培養神経細胞の抗体供給した後細胞表面および内在化タンパク質の異なる標識化
不連続ショ糖勾配を用いてシナプス形質膜とシナプス後肥厚タンパク質の調製
細胞内のCaの蛍光と生物発光イメージング<sup> 2+</sup>高齢者における海馬ニューロン
蛍光プローブを用いた培養ニューロンにおけるエンドソームおよびリソソームの動力学の定量
Read Article