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Superando indiferença em Encefalomielite autoimune experimental (EAE) linhagens mais resistentes por transferência adotiva e desafio antigênico
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Chapters
- 00:05Title
- 01:28Preparing CFA-emulsified Antigen
- 03:08Immunization of Donor Mice
- 03:51Isolation of Lymph Node Cells after Immunization and Re-stimulation with Antigen
- 06:07Harvest of Re-stimulated Lymph Node Cells and Adoptive Transfer
- 06:47Antigenic Challenge of Recipient Mice and Development of Disease
- 07:31Results: Kinetics of Disease Progression Following Immunization, Adoptive Transfer and Challenge
- 07:58Conclusion
Linhagens determinados são capazes de resistir a indução de encefalomielite autoimune experimental (EAE) com proteína básica de mielina. Descrito aqui é um simples protocolo de imunização que reverte a apatia e induz a doença paralítica em vários típicos EAE manchas resistentes do mouse.
Tags
Experimental Autoimmune EncephalomyelitisEAEMouse StrainsAdoptive TransferAntigenic ChallengeCentral Nervous SystemMononuclear CellsParalytic DiseaseMultiple SclerosisGenetic ControlC57BL/6 MiceMyelin Basic ProteinAntigen ProcessingAntigen PresentationEncephalitogenic T Cell ClonesCellular Adoptive Transfer System
