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Superar la falta de respuesta en encefalomielitis autoinmune experimental (EAE) cepas de ratones resistentes a través de transferencia adoptiva y la estimulación antigénica
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
DOI:

08:38 min

April 09, 2012

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Chapters

  • 00:05Title
  • 01:28Preparing CFA-emulsified Antigen
  • 03:08Immunization of Donor Mice
  • 03:51Isolation of Lymph Node Cells after Immunization and Re-stimulation with Antigen
  • 06:07Harvest of Re-stimulated Lymph Node Cells and Adoptive Transfer
  • 06:47Antigenic Challenge of Recipient Mice and Development of Disease
  • 07:31Results: Kinetics of Disease Progression Following Immunization, Adoptive Transfer and Challenge
  • 07:58Conclusion

Summary

Automatic Translation

Ciertas cepas de ratón son capaces de resistir la inducción de la encefalomielitis autoinmune experimental (EAE) con proteína básica de mielina. Aquí se describe es un protocolo de inmunización simple que invierte la falta de respuesta e induce la enfermedad paralítica en varios típicas manchas de EAE de ratón resistentes.

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