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Kabul edilmiş Transfer ve antijenik Challenge Deneysel Otoimmün Encephalomyelitis (EAE) Dayanıklı Fare Suşlarında yanıtsızlık Aşmak
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
DOI:

08:38 min

April 09, 2012

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Chapters

  • 00:05Title
  • 01:28Preparing CFA-emulsified Antigen
  • 03:08Immunization of Donor Mice
  • 03:51Isolation of Lymph Node Cells after Immunization and Re-stimulation with Antigen
  • 06:07Harvest of Re-stimulated Lymph Node Cells and Adoptive Transfer
  • 06:47Antigenic Challenge of Recipient Mice and Development of Disease
  • 07:31Results: Kinetics of Disease Progression Following Immunization, Adoptive Transfer and Challenge
  • 07:58Conclusion

Summary

Automatic Translation

Bazı fare suşlar miyelin temel protein olan deneysel otoimmün ensefalomyelit (EAE) indüksiyon karşı edebiliyoruz. Burada anlatılan yanıtsızlık tersine çevirir ve birkaç tipik EAE dirençli fare lekeler paralitik hastalığın neden basit bir bağışıklama protokoldür.

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