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Biology
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化学阻断抗体芯片复用高通量的复杂样品中特定蛋白糖基化的剖析
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JoVE Journal
Biology
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Please note that all translations are automatically generated.
Click here for the English version.
化学阻断抗体芯片复用高通量的复杂样品中特定蛋白糖基化的剖析
DOI:
10.3791/3791-v
•
13:21 min
•
May 04, 2012
•
Chen Lu
,
Joshua L. Wonsidler
,
Jianwei Li
,
Yanming Du
,
Timothy Block
,
Brian Haab
,
Songming Chen
1
Institute for Hepatitis and Virus Research
,
2
Department of Microbiology and Immunology
,
Thomas Jefferson University
,
3
Drexel University College of Medicine
,
4
Van Andel Research Institute
Chapters
00:05
Title
02:19
Printing the Antibody Microarray
03:58
Chemically Blocking the Antibody Microarray
06:06
Blocking Non-specific Binding with BSA
06:47
Wax Imprinting
07:11
Applying Serum Samples
08:19
Applying Biotinylated Glycan-binding Protein
09:00
Applying Dye-labeled NeutrAvidin and Fluorescence Detection
10:03
Data Extraction and Analysis
12:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这项研究中,我们描述了一个复用高通量抗体芯片与外源凝集素的检测方法,可以在特定的蛋白质糖基化分析改进协议。该协议提供了新的可靠的试剂和显着降低,相比以前的程序的时间,成本和实验室设备的要求。
Tags
Chemically-blocked Antibody Microarray
Multiplexed High-throughput Profiling
Specific Protein Glycosylation
Complex Samples
Glycan Binding Protein Detection
Post-translational Modification
Physical Properties Of Proteins
Chemical Properties Of Proteins
Biological Properties Of Proteins
Disease Progression
Malignant Transformation
Early Detection Biomarkers
Cancer Biomarkers
Practical Method
Laboratory Settings
Clinical Settings
Chemically Blocked Antibody Microarray
Glycan-binding Protein Detection
Time Reduction
Cost Reduction
Lab Equipment Requirements
Article
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