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化学阻断抗体芯片复用高通量的复杂样品中特定蛋白糖基化的剖析
JoVE Journal
Biology
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JoVE Journal Biology
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
DOI:

13:21 min

May 04, 2012

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Chapters

  • 00:05Title
  • 02:19Printing the Antibody Microarray
  • 03:58Chemically Blocking the Antibody Microarray
  • 06:06Blocking Non-specific Binding with BSA
  • 06:47Wax Imprinting
  • 07:11Applying Serum Samples
  • 08:19Applying Biotinylated Glycan-binding Protein
  • 09:00Applying Dye-labeled NeutrAvidin and Fluorescence Detection
  • 10:03Data Extraction and Analysis
  • 12:08Conclusion

Summary

Automatic Translation

在这项研究中,我们描述了一个复用高通量抗体芯片与外源凝集素的检测方法,可以在特定的蛋白质糖基化分析改进协议。该协议提供了新的可靠的试剂和显着降低,相比以前的程序的时间,成本和实验室设备的要求。

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