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基因分型的植物和动物样品,恕不另行DNA的纯化
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Genotyping of Plant and Animal Samples without Prior DNA Purification
DOI:

11:00 min

September 24, 2012

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Chapters

  • 00:05Title
  • 01:48Genotyping of Arabidopsis Plant Individuals with Direct Protocol
  • 04:23Amplifying Specific DNA Fragments from 15-year-old Oak Leaves using the Dilution Protocol
  • 05:50Genotyping of Transgenic Mice using the Dilution Protocol
  • 07:05Results: Direct PCR Analysis
  • 10:30Conclusion

Summary

Automatic Translation

这里提出的直接PCR方法便于直接从少量的未精制的植物和动物组织的PCR扩增。

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