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Genotypebestemmelse af plante-og dyrearter Prøver uden forudgående DNA-oprensning
JoVE Journal
Biology
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JoVE Journal Biology
Genotyping of Plant and Animal Samples without Prior DNA Purification

Genotypebestemmelse af plante-og dyrearter Prøver uden forudgående DNA-oprensning

DOI:
10.3791/3844-v

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11:00 min

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September 24, 2012

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, , , ,
1Thermo Scientific Molecular Biology Products,Thermo Fisher Scientific

Chapters

  • 00:05Title
  • 01:48Genotyping of Arabidopsis Plant Individuals with Direct Protocol
  • 04:23Amplifying Specific DNA Fragments from 15-year-old Oak Leaves using the Dilution Protocol
  • 05:50Genotyping of Transgenic Mice using the Dilution Protocol
  • 07:05Results: Direct PCR Analysis
  • 10:30Conclusion

Summary

Automatic Translation

Automatic Translation

Den direkte PCR strategi, der fremlægges her, letter PCR-amplifikation direkte fra små mængder af urenset plante-og animalsk væv.

Tags

GenotypingPlant SamplesAnimal SamplesDNA PurificationDirect PCR ApproachThermo Scientific PhusionThermo Scientific Phire DNA PolymerasesPCR-based Target DNA DetectionPlant ResearchGenotype AnalysisTransgenesDNA IsolationThermo Scientific Phire Plant Direct PCR KitArabidopsis Plant LeavesCleaved Amplified Polymorphic Sequence Analysis (dCAPS)Single Nucleotide Polymorphisms (SNPs)Restriction Endonuclease DigestionPhenolic Compounds

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