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Immunology and Infection
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En TIRF Mikroskopi Technique for i realtid, Samtidig Billeddannelse af TCR og dens associerede signalproteiner
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

En TIRF Mikroskopi Technique for i realtid, Samtidig Billeddannelse af TCR og dens associerede signalproteiner

DOI:
10.3791/3892-v

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16:10 min

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March 22, 2012

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, ,
1Laboratory of Cellular and Molecular Immunology,National Institute of Allergy and Infectious Diseases, National Institutes of Health

Chapters

  • 00:05Title
  • 02:59Transfection Using Amaxa Mouse T Cell Nucleofector Kit
  • 06:07TIRF Setup
  • 10:24Imaging
  • 13:19Representative Results
  • 14:54Conclusion

Summary

Automatic Translation

Automatic Translation

Den opdeling af proteiner enten i plasmamembranen eller ind i intracellulære steder er en regulerende mekanisme, der kan have stor indflydelse signalanlæg resultater, og derfor til at forstå signalere er det vigtigt at studere den rumlige og tidsmæssige opførsel af de involverede proteiner. Vi beskriver her en TIRF mikroskopi system til at undersøge signaltransduktion i T-celler, men er bredt anvendelig.

Tags

TIRF MicroscopyReal-time ImagingTCRSignaling ProteinsAntigen Presenting CellsPMHC ProteinsTCR ComplexCD3 DimersSrc Family KinaseLckSyk Family KinaseZap70Adapter ProteinsLATSLP76Proximal Signaling ComplexCalcium-dependent Transcription Factor ActivationGene Expression ProgramsT Cell DifferentiationAntigen PotencyCo-stimulatory ReceptorsCo-inhibitory ReceptorsChemokine ReceptorsCytokine Receptors

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