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研究蛋白质折叠的微流体混合器
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Microfluidic Mixers for Studying Protein Folding
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研究蛋白质折叠的微流体混合器

DOI:
10.3791/3976-v

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12:42 min

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April 10, 2012

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, , , ,
1Department of Physics and Astronomy,Michigan State University, 2Department of Mechanical Engineering,Hong Kong University of Science and Technology, 3Center for Biophotonics,University of California, Davis

Chapters

  • 00:05Title
  • 01:05Fabrication of Microfluidic Mixing Chips
  • 05:46Mounting and Loading Chips
  • 07:35Data Collection
  • 09:55Results: Protein Folding Analysis using Microfluidic Mixers
  • 12:20Conclusion

Summary

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Automatic Translation

在这项工作中，我们解释的制造和使用微流体混合器混合〜8微秒的两个解决方案的能力。我们也证明了这些混频器的使用与光谱紫外荧光检测和荧光共振能量转移（FRET）的使用。

Tags

Microfluidic MixersProtein FoldingNative ConformationFolding KineticsStopped-flow MixersMicrofluidic MixerDilution RateLaminar FlowTurbulenceNumeric SimulationReynolds NumbersAqueous SolutionsMicron Scale GeometriesHard SubstrateChannelsOptical AccessLinear Flow RatesProtein ConsumptionProtein ConcentrationTryptophan FluorescenceFRET

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