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Neuroscience
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代的永生化小鼠脑微血管内皮细胞株作为<em在体外</em血脑屏障型号
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an
In Vitro
Blood Brain Barrier Model
Please note that all translations are automatically generated.
Click here for the English version.
代的永生化小鼠脑微血管内皮细胞株作为<em在体外</em血脑屏障型号
DOI:
10.3791/4022-v
•
09:36 min
•
August 29, 2012
•
Malgorzata Burek
,
Ellaine Salvador
,
Carola Y. Förster
1
Klinik und Poliklinik für Anästhesiologie
,
University of Wurzburg
Chapters
00:05
Title
01:06
Isolation of Brain Microvessels
04:35
Immortalizing the Brain Microvascular Endothelial Cells
07:22
Cultivating the Brain Microvascular Endothelial Cells
08:13
Results: Representative Images of cEND Cells
09:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此方法描述了如何从小鼠脑微血管内皮细胞隔离和不朽。我们描述了一个一步一步的协议,从同质化的脑组织,消化步骤,播种和永生化的细胞。通常情况下,大约需要五周获得一个同质的,永生的微血管内皮细胞系。
Tags
Immortalized Cell Line
Murine Brain Microvascular Endothelial Cells
In Vitro Blood Brain Barrier Model
Paracellular Barriers
Blood-brain Barrier (BBB)
Astrocyte End-feet
Pericytes
Basal Membrane
Brain Parenchyma
In Vitro BBB Models
Primary Cell Preparations
Immortalized EC Lines
Neonatal Mouse Brain
Reagents And Solutions
Homogenous Endothelial Cell Line
CEND 4
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