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An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers
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JoVE Journal Neuroscience
An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

DOI:
10.3791/57038-v

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09:35 min

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February 26, 2018

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, , ,
1Institute of Neurology (Edinger Institute),Goethe University Hospital, 2Pharmazentrum Frankfurt, Institute for General Pharmacology and Toxicology,Goethe University Hospital

Chapters

  • 00:04Title
  • 01:15Administration of Tracers
  • 02:01Blood Collection and Cardiac Perfusion
  • 03:36Organ Collection and Storage
  • 05:01Homogenization and Centrifugation
  • 06:38Immunofluorescence Visualization of Tracer
  • 07:59Results: Tracer Clearance at Short and Long Circulation Times
  • 08:51Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a mouse brain vascular permeability assay using intraperitoneal injection of fluorescent tracers followed by perfusion that is applicable to animal models of blood-brain barrier dysfunction. One hemi-brain is used for assessing permeability quantitatively and the other for tracer visualization/immunostaining. The procedure takes 5 - 6 h for 10 mice.

Tags

Keywords: In VivoBlood-brain BarrierPermeabilityFluorescent TracersMiceNeurological DisordersBrain EdemaBlood-brain Barrier DysfunctionCardiac PerfusionFluorometryFluorescence Microscopy

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