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Chapters
- 00:05Title
- 03:31DNA Template Preparation, In vitro Transcription and Translation
- 05:34Isolation of Nascent Polypeptides and Analysis by Tris-tricine SDS PAGE
- 07:17Representative RNC Results
- 07:57Conclusion
Vi beskriver her en teknik, der nu rutinemæssigt anvendes til at isolere stabilt bundet ribosom vordende kæde-komplekser (RNC'er). Denne teknik udnytter den erkendelse, at en 17 aminosyre langt SecM "arrest sekvens" kan standse translation forlængelse i en prokaryot (<em> E. coli</em>) System, når det indsættes i (eller fusioneret til C-terminus) af praktisk taget hvilket som helst protein.
Tags
SecM Arrest SequenceRibosome Bound PolypeptidesCo-translational FoldingIsolation TechniqueRNCs (ribosome-nascent Chain Complexes)Protein Folding PathwayConformational AnalysisSecM ProteinSecretion MonitorSecA ATPaseStalling SequencePeptidyl-glycyl-tRNAC-terminal RegionPolypeptide Chain ElongationIn Vivo Studies
