A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:26Preparation and Setup
- 02:27Pancreas Dissection
- 06:08Spleen, Lymph Node, and Duodenal Mucosa Dissection
- 07:09Results: Pancreas Weights by Region
- 07:25Conclusion
Este video muestra la disección de un procedimiento para la tramitación de páncreas humano en los formatos de almacenamiento. Orientación anatómica se mantiene a lo largo de las regiones pancreáticas para permitir la definición de la composición islote regional y densidad.
Tags
Collection ProtocolHuman PancreasDissectionSampling ProcedureNetwork For Pancreatic Organ Donors With Diabetes (nPOD)StandardizeCadaveric Organ DonorsPancreas RegionsHeadBodyTailSerial Transverse SectionsFixed ParaffinFresh Frozen BlocksSnap Frozen Sample PreparationsRNAse InhibitorsDNARNAProtein IsolationPancreas Dissection ProcedureAnatomical OrientationEndocrine Cell HeterogeneityIslet CompositionSizeNumbersInsulin-containing β-cellsGlucagon-containing α-cellsSomatostatin-containing δ-cellsPancreatic Polypeptide-containing PP CellsGhrelin-containing Epsilon CellsUncinate RegionDevelopmental Differences
