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High-throughput Oprensning af Affinity-mærkede rekombinante proteiner
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Biology
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JoVE Journal Biology
High-throughput Purification of Affinity-tagged Recombinant Proteins

High-throughput Oprensning af Affinity-mærkede rekombinante proteiner

DOI:
10.3791/4110-v

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07:44 min

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August 26, 2012

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,
1Department of Life Sciences,Imperial College London

Chapters

  • 00:05Title
  • 01:27High-throughput Growth of Bacterial Cultures
  • 02:38Robotic Purification of Recombinant Proteins
  • 04:06Protein Purification
  • 06:32Representative Protein Purification Results
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en fremgangsmåde til affinitets-taggede oprensning af rekombinante proteiner ved hjælp af væske-handling robot. Denne metode er generelt anvendelig for små oprensning af opløselige His-mærkede proteiner i en high-throughput format.

Tags

High-throughput PurificationAffinity-tagged Recombinant ProteinsX-ray CrystallographyBiochemical AnalysesStructure/function RelationshipsLarge-scale Mutagenesis StrategiesAmino Acids SubstitutionGain/loss-of-function PhenotypesCatalytic ActivityProtein StabilityProtein-protein Interaction SpecificityControlled And Reproducible MannerHigh-throughput StrategiesRobotic Liquid-handling PlatformsHis-tagged Recombinant ProteinsBasal Transcription Machinery

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