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Journal
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Immunology and Infection
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反向遗传学感染小鼠诺沃克病毒介导的恢复
JoVE Journal
Immunology and Infection
Author Produced
This content is Free Access.
JoVE Journal
Immunology and Infection
Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
Please note that all translations are automatically generated.
Click here for the English version.
反向遗传学感染小鼠诺沃克病毒介导的恢复
DOI:
10.3791/4145-v
•
13:48 min
•
June 24, 2012
•
Armando Arias*
1
,
Luis Ureña*
1
,
Lucy Thorne
,
Muhammad A. Yunus
,
Ian Goodfellow
1
Section of Virology
,
Imperial College London
Chapters
00:05
Title
01:34
Procedure Overview
03:16
RNA Transcription and Capping for the Recovery of Infectious MNV
06:27
MNV Recovery by RNA Electroporation into Raw Cells
08:25
Recovery by Lipofection into BSR-T7 Cells
10:07
Direct Recovery of Infectious MNV from cDNA in Cells Expressing T7 RNA Polymerase
12:20
Representative Results
Summary
Automatic Translation
English (Original)
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Automatic Translation
诺如病毒肠胃炎尚未分子技术是表征的主要原因仍然是相对较新的。在这里,我们报告两个不同的反向遗传学方法对小鼠诺沃克病毒(MNV),本属的唯一成员,可在细胞培养繁殖的高效回收。
Tags
Reverse Genetics
Recovery
Infectious
Murine Norovirus
Human Noroviruses
Gastroenteritis
Outbreaks
Hospitals
Antiviral Approaches
Cell Culture
Propagation
Genomic RNA Molecules
Open Reading Frames (ORFs)
Non-structural Proteins
Capsid Proteins
Virulence Factor (VF1)
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